Product: Anti-CIDE-A (mouse, C-terminus), Polyclonal
Cat. No: PC-037 (100 μg)
Background:
Apoptosis is related to many diseases and
induced by a family of cell death receptors and
their ligands. Cell death signals are transduced
by death domain containing adapter molecules
and members of the caspase family of proteases.
These death signals finally cause the
degradation of chromosomal DNA by activated
DNase. DFF45/ICAD has been identified as
inhibitor of caspase activated DNase DFF40
/CAD. DFF45 related proteins CIDE-A and
CIDE-B (for cell death-inducing DFF-like effector
A and B) were recently identified. CIDE contains
a new type of domain termed CIDE-N, which
has high homology with the regulatory domains
of DFF45/ICAD and DFF40/CAD. Expression of
CIDE-A induces DNA fragmentation and
activates apoptosis, which is inhibited by DFF45.
CIDE-A is a DFF45-inhibitable effector that
promotes cell death and DNA fragmentation.
CIDE-A is expressed in many tissues.
Specificity:
Binds CIDE-A. Antibody has no cross activity to
CIDE-B.
Positive control: Tissue lysate of murine heart.
An approximately 25 kDa band should be
detected.
Species Reactivity:
Mouse, others not tested.
Ig Isotype:
Rabbit IgG
Immunogen:
A peptide corresponding to amino acids 200 to
214 of mouse CIDE-A.
Format:
Immunoaffinity chromatography purified IgG,
100 μg in 200 μl of PBS containing 0.02%
sodium azide.
Storage and Stability:
Stable for at least one year when stored at 4°C.
Applications and Suggested Dilutions:

Western blot: Suggested dilution: 1:500 to
1:1000.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.